Quick freezing of mouse embryos by direct plunge into liquid nitrogen vapor: effects of sugars.

Instructions for use Title QUICK FREEZING OF MOUSE EMBRYOS BY DIRECT PLUNGE INTO LIQUID NITROGEN VAPOR : EFFECTS OF SUGARS

Effects of various cryoprotectants on the survival of mouse embryos cryopreserved by the quick freezing method.

The effects of concentrations of glycerol, ethylene glycol or dimethylsulphoxide (DMSO) in the presence of either 0.25 M lactose or sucrose on the post-thaw survival of mouse quickly-frozen compacted morulae were studied. In this method, the embryos were directly frozen in liquid nitrogen (LN2) vapor at approximately -170 degrees C for 2 min before being plunged into LN2. High survival rates of...

P-26: The Effect of Zygote and 2-cell Development Stages on Vitrification Process of Mouse Embryo

Background: While it is possible to routinely cryopreserve embryos from several mammalian species, the cryopreservation of embryos has largely been limited by their high sensitivity to chilling injury. Many factors such as the stage of embryonic development, cryoprotectant toxicity, the composition of the vitrification solution and cooling and warming rates can influence survival of embryos aft...

Effect of freezing rate and exposure time to cryoprotectant on the development of mouse pronuclear stage embryos.

BACKGROUND The effects of exposure time (20 versus 45 s) to a high concentration of cryoprotectant (7.0 mol/l ethylene glycol with 0.5 mol/l sucrose) and freezing rates (1200-10 300 degrees C/min) during rapid freezing of mouse pronuclear stage embryos on survival and development to blastocysts were investigated. Different freezing rates were achieved by directly plunging the straws (rapid free...

An attempt of cryopreservation of mouse embryos at the ACTREC laboratory animal facility in India.

Cryopreservation is the long-term storage of viable cells/tissue in liquid nitrogen. The present study was conducted to freeze 8-cell- to morula-stage mouse embryos from the ACTREC Laboratory Animal Facility using a "slow freezing and fast revival" method. In all, 4,088 embryos were collected from 495 donor female mice of ten different strains. An average recovery of 8 embryos per donor mouse w...